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Comparative protein expression in different strains of the bloom-forming cyanobacterium Microcystis aeruginosa

机译：形成水华的蓝藻微囊藻不同菌株中的比较蛋白表达

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摘要

Toxin production in algal blooms presents a significant problem for the water industry. Of particular concern is microcystin, a potent hepatotoxin produced by the unicellular freshwater species Microcystis aeruginosa. In this study, the proteomes of six toxic and nontoxic strains of M. aeruginosa were analyzed to gain further knowledge in elucidating the role of microcystin production in this microorganism. This represents the first comparative proteomic study in a cyanobacterial species. A large diversity in the protein expression profiles of each strain was observed, with a significant proportion of the identified proteins appearing to be strain-specific. In total, 475 proteins were identified reproducibly and of these, 82 comprised the core proteome of M. aeruginosa. The expression of several hypothetical and unknown proteins, including four possible operons was confirmed. Surprisingly, no proteins were found to be produced only by toxic or nontoxic strains. Quantitative proteome analysis using the labelfree normalized spectrum abundance factor approach revealed nine proteins that were differentially expressed between toxic and nontoxic strains. These proteins participate in carbon-nitrogen metabolism and redox balance maintenance and point to an involvement of the global nitrogen regulator NtcA in toxicity. In addition, the switching of a previously inactive toxin-producing strain to microcystin synthesis is reported.
机译：藻华中的毒素产生对水工业提出了重大问题。特别令人关注的是微囊藻毒素，一种由单细胞淡水物种铜绿微囊藻产生的有效肝毒素。在这项研究中，对六种铜绿假单胞菌的有毒和无毒菌株的蛋白质组进行了分析，以进一步阐明微囊藻毒素在该微生物中的作用。这代表了在蓝细菌物种中的第一个比较蛋白质组学研究。在每个菌株的蛋白质表达谱中观察到很大的差异，其中很大比例的鉴定出的蛋白质似乎是菌株特异性的。总共可鉴定出475种蛋白质，其中82种是铜绿假单胞菌的核心蛋白质组。证实了几种假设的和未知的蛋白的表达，包括四个可能的操纵子。令人惊讶地，没有发现仅由毒性或无毒菌株产生蛋白质。使用无标记归一化谱丰度因子方法进行的蛋白质组定量分析显示，有毒和无毒菌株之间差异表达的九种蛋白质。这些蛋白质参与碳氮代谢和氧化还原平衡维持，并指出全球氮调节剂NtcA参与毒性。另外，据报道，以前无活性的毒素产生菌株转换为微囊藻毒素合成。

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